Strategies for signal amplification in nucleic acid detection |
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Authors: | S Calin Andras J Brian Power Edward C Cocking Michael R Davey |
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Affiliation: | (1) Plant Science Division, School of Biosciences, University of Nottingham, NG7 2RD University Park, Nottingham, UK;(2) Faculty of Biology and Geology, Ecology and Genetics Department, “Babes-Bolyai” University, Str. Clinicilor 5-7, 3400 Cluj-Napoca, Romania |
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Abstract: | Many aspects of molecular genetics necessitate the detection of nucleic acid sequences. Current approaches involving target
amplification (in situ PCR, Primed in situ Labeling, Self-Sustained Sequence Replication, Strand Displacement Amplification), probe amplification (Ligase Chain Reaction,
Padlock Probes, Rolling Circle Amplification) and signal amplification (Tyramide Signal Amplification, Branched DNA Amplification)
are summarized in the present review, together with their advantages and limitations. |
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Keywords: | Signal amplification nucleic acid detection in situ hybridization in situ PCR primed in situ labeling Self-Sustained Sequence Replication Strand Displacement Amplification Ligase Chain Reaction Padlock Probes Rolling Circle Amplification Tyramide Signal Amplification Branched DNA Amplification |
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