首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Brain injury induces cholesterol 24-hydroxylase (Cyp46) expression in glial cells in a time-dependent manner
Authors:Kosara Smiljanic  Irena Lavrnja  Aleksandra Mladenovic Djordjevic  Sabera Ruzdijic  Mirjana Stojiljkovic  Sanja Pekovic  Selma Kanazir
Institution:(1) Department of Neurobiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Blvd. Despot Stefana 142, Belgrade, Serbia;
Abstract:Maintaining the cholesterol homeostasis is essential for normal CNS functioning. The enzyme responsible for elimination of cholesterol excess from the brain is cholesterol 24-hydroxylase (Cyp46). Since cholesterol homeostasis is disrupted following brain injury, in this study we examined the effect of right sensorimotor cortex suction ablation on cellular and temporal pattern of Cyp46 expression in the rat brain. Increased expression of Cyp46 at the lesion site at all post injury time points (2, 7, 14, 28 and 45 days post injury, dpi) was detected. Double immunofluorescence staining revealed colocalization of Cyp46 expression with different types of glial cells in time-dependent manner. In ED1+ microglia/macrophages Cyp46 expression was most prominent at 2 and 7 dpi, whereas Cyp46 immunoreactivity persisted in reactive astrocytes throughout all time points post-injury. However, during the first 2 weeks Cyp46 expression was enhanced in both GFAP+ and Vim+ astrocytes, while at 28 and 45 dpi its expression was mostly associated with GFAP+ cells. Pattern of neuronal Cyp46 expression remained unchanged after the lesion, i.e. Cyp46 immunostaining was detected in dendrites and cell body, but not in axons. The results of this study clearly demonstrate that in pathological conditions, like brain injury, Cyp46 displayed atypical expression, being expressed not only in neuronal cells, but also in microglia and astrocytes. Therefore, injury-induced expression of Cyp46 in microglial and astroglial cells may be involved in the post-injury removal of damaged cell membranes contributing to re-establishment of the brain cholesterol homeostasis.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号