Determination of the expression time and the dose-response relationship for mutations at the HGPRT (hypoxanthine-guanine-phosphoribosyl transferase) locus induced by X-irradiation in human diploid skin fibroblasts |
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Authors: | YCEM de Ruijter JWIM Simons |
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Institution: | Department of Radiation Genetics and Chemical Mutagenesis, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden The Netherlands |
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Abstract: | The lenth of the expression time for mutants resistant to 8-azaguanine or 6-thioguanine induced by X-rays was determined in human diploid skin fibrobalsts. The cells were seeded in the selective medium over a period of 14 days after treatment. Direct expression of at least a part of the mutants was observed at day 0, and an increase of the mutant frequency over the entire cultivation period appeared to be due to spontaneous mutation.The dose-response relationship does not appear to deviate from linearity. The mutation rate per R had a mean value of 2.1 × 10?7 which is about twice the value of the mutation rate found in rodent cells for the same locus. |
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