Comparison of rat and guinea pig as sources of the S9 fraction in the salmonella/mammalian microsome mutagenicity test |
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| Authors: | Robert S.U. Baker Antonio M. Bonin Ieva Stupans Gerald M. Holder |
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| Affiliation: | 1. School of Public Health and Tropical Medicine (Australian Department of Health) New South Wales 2006, Australia;2. Department of Pharmacy, University of Sydney, New South Wales 2006, Australia |
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| Abstract: | A highly significant enhancement of mutagenicity occurs with 11 polycyclic aromatic hydrocarbons when 3-methylcholanthrene-induced guinea pig liver S9 is substituted for Aroclor-induced rat liver S9 in the Ames test. The use of MC-induced guinea pig liver S9 is particularly valuable for detecting the weak mutagenicity of benz[c]acridine, which is barely positive in a standard Ames assay. However, anthracene and phenanthrene, which are generally considered not to be carcinogens, remain non-mutagenic for strain TA100. This enhancement of mutagenicity does not correlate with arylhydrocarbon hydroxylase activities of the various liver preparations and does not apply to certain other non-PAH mutagens, including β-naphthylamine, aflatoxin B1 and 4-dimethylaminoazobenzene. |
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| Keywords: | AAF 2-acetylaminofluorene AHH arylhydrocarbon hydroxylase MC 3-methyl-cholanthrene PAH polycyclic aromatic hydrocarbon(s) S9 To whom correspondence should be addressed. |
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