Chromosomal radiosensitivity of human leucocytes in relation to sampling time |
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Authors: | Paul PW van Buul AT Natarajan |
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Institution: | 1. Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratories, Wassenaarseweg 72, 2333 AL Leiden The Netherlands;2. J.A. Cohen Institute, Interuniversity Institute of Radiation Protection and Radiophathology, Leiden The Netherlands |
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Abstract: | Frequencies of chromosomal aberrations after irradiation with X-rays of peripheral blood lymphocytes in vitro were determined at different times after initiation of cultures. In each culture, the kinetics of cell multiplication was followed by using BrdU labelling and differential staining of chromosomes.The results indicate that the mixing up of first and second cell cycle cells at later sampling times cannot explain the observed variation in the frequencies of chromosomal aberrations but that donor-to-donor variation is a predominant factor influencing yields of aberrations. The condition of a donor seems to be most important because repeats on the same donor also showed marked variability. |
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