Biochemical characterization of six trisomics of grain sorghum,Sorghum bicolor (L.) Moench |
| |
Authors: | H W Suh D R Goforth Bryce A Cunningham G H Liang |
| |
Institution: | (1) Department of Agronomy, Kansas State University, Manhattan, Kansas;(2) USDA Grain Marketing Research Center, Manhattan, Kansas;(3) Department of Biochemistry, Kansas State University, Manhattan, Kansas |
| |
Abstract: | To determine protein differences of grain sorghum disomics and trisomics, we analyzed leaf extracts from six trisomics and a disomic control by disc gel, gel isoelectric focusing, and SDS gel electrophoresis. Based on the number and position of protein bands revealed by Commassie blue staining, the disomic control could be differentiated from the trisomics, and trisomics could be shown to differ among themselves in most cases. SDS gels revealed the most protein bands, followed by isoelectric focusing and disc gel. However, disc gel electrophoresis was the simplest technique of the three and was just as effective in identifying trisomics and differentiating trisomics from the disomic control.Contribution 1596-j, Department of Agronomy, and 182-j, Department of Biochemistry, Kansas State University, Manhattan, Kansas. |
| |
Keywords: | disc electrophoresis sorghum trisomics SDS gel electrophoresis isoelectric focusing |
本文献已被 SpringerLink 等数据库收录! |
|