| 生物组织背向SHG和TPEF的影响因素 |
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| 引用本文： | 刘嫩容,邱怡申. 生物组织背向SHG和TPEF的影响因素[J]. 激光生物学报, 2007, 16(5): 620-625 |
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| 作者姓名： | 刘嫩容 邱怡申 |
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| 作者单位： | 福建师范大学物理与光电信息科技学院、医学光电科学与技术教育部重点实验室,福建,福州,350007;福建师范大学物理与光电信息科技学院、医学光电科学与技术教育部重点实验室,福建,福州,350007 |
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| 摘 要： | 由于生物组织的复杂性和多样性,以及样品制备等因素的影响,实验观察到的生物组织的背向二次谐波(second harmonic generation,SHG)和双光子激发荧光(two-photon excitation fluorescence,TPEF)效应的差异较大。以鼠尾组织作为实验对象,共40个切片,分为横向和纵向、HE染色和未染色四组,采用飞秒激光器作为激发光,用双光子激光扫描共焦显微镜(two-photon laser scanning confocal microscope,TPLSCM)观察和分析了样品在不同的制备方式、激发波长、激发功率、扫描深度等条件下的背向SHG和TPEF的变化曲线,讨论和比较了生物组织的背向SHG和TPEF的影响因素以及二者之间的异同,并尝试对实验现象做出了一定的解释。
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| 关 键 词： | 生物组织 背向二次谐波 双光子激发荧光 |
| 文章编号： | 1007-7146（2007）05-0620-06 |
| 收稿时间： | 2006-10-18 |
| 修稿时间： | 2007-03-23 |
Study of Various Effects on Backward SHG and TPEF in Biotissue |
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| LIU Nen-rong,QIU Yi-shen. Study of Various Effects on Backward SHG and TPEF in Biotissue[J]. ACTA Laser Biology Sinica, 2007, 16(5): 620-625 |
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| Authors: | LIU Nen-rong QIU Yi-shen |
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| Affiliation: | School of Physics and OptoElectronics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, Fujian, China |
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| Abstract: | Because of the complexities and varieties of biological tissues,and the influences of sample preparation,the backward SHG and TPEF of different tissues are different.In this thesis,rat-tails were used as experiment objects,there are 40 slices including transverse and longitudinal,HE-dyed and undyed.Excited by femtosecond laser,the changes of backward SHG and TPEF versus sample preparation,excitation wavelength,excitation power,penetration depth and so on were observed by two-photon laser scanning confocal microscope(TPLSCM),effect factors of backward SHG and TPEF and differences of both were discussed and compared,and related comments were also presented. |
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| Keywords: | biological tissue backward second harmonic generation(SHG) two-photon excitation fluorescence(TPEF) |
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