The fate of mitochondria in Ibex-hirus reconstructed early embryos |
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Authors: | Jiang Yan Liu Shu-Zhen Zhang Yan-Ling Jiang Man-Xi Sun Qing-Yuan Chen Da-Yuan |
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Institution: | State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Sciences, Beijing 100080, China. |
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Abstract: | Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages. |
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