Gonadotropin stimulated androgen secretion of rainbow trout (Salmo gairdneri Richardson) testis in vitro

1. The secretion of five androgens was quantified from trout testes under GTH-stimulation in vitro before and after the onset of milt production, and a general increase of basal and GTH stimulated androgen secretion was recorded during this period. 2. 11-Ketotestosterone, testosterone, and in spermiating males, 11 beta-hydroxytestosterone as well showed GTH dependent concentration increases, while androstenetrione and 11 beta-hydroxyandrostendione were found in highly variable amounts. 3. 17 beta-Hydroxyandrogen glucuronides in the medium (with the exception of testosterone) and tissue androgens were by far exceeded by the free androgens in the medium.