A high melting cis-[Pt(NH3)2[d(GpG)]]adduct of a decanucleotide duplex |
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| Authors: | B van Hemelryck E Guittet G Chottard J P Girault F Herman T Huynh-Dinh J Y Lallemand J Igolen J C Chottard |
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| Affiliation: | 1. CAS High Magnetic Field Laboratory, Department of Chemistry, University of Science and Technology of China, Hefei Anhui 230026, China;2. Department of Chemistry, University of Bari “A. Moro”, via E. Orabona 4, 70125 Bari, Italy;3. Department of Chemistry, University of Bergen, Norway;1. Computational Biomedicine, Institute for Advanced Simulation IAS-5 and Institute of Neuroscience and Medicine INM-9, Forschungszentrum Jülich, Jülich, Germany;2. Jülich Supercomputing Centre (JSC), Forschungszentrum Jülich, Jülich, Germany;3. Department of Oncology, Hematology and Stem Cell Transplantation, RWTH Aachen University, Aachen, Germany;4. Department of Chemistry, University of Bari “Aldo Moro”, via Edoardo Orabona 4, 70125 Bari, Italy;1. Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Monteroni, I-73100 Lecce, Italy;2. Neuropathology Unit, Institute of Experimental Neurology and Division of Neuroscience, IRCCS San Raffaele Scientific Institute, via Olgettina 60, 20132 Milan, Italy;1. Fakultät Chemie und Chemische Biologie (CCB), Technische Universität, Dortmund 44221 Dortmund, Germany;2. Department of Chemistry, University of Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland;3. Department of Chemistry, Inorganic Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland |
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| Abstract: | The [cis-Pt(NH3)2(d(GCCGGATCGC)-N7(4), N7(5))]-d(GCGATCCGGC) duplex has been prepared with Tm = 49 degrees C (vs 58 degrees C for the unplatinated form). NMR of the ten observable imino protons supports a kinked structure with intact base pairing of the duplex on the 3'-side of the d(GpG).cis-Pt chelate (relative to the platinated strand) The modification of the B-DNA type CD spectrum, due to the platinum chelate, is comparable to that observed for the platination (at a 0.05 Pt:base ratio) of the Micrococcus Lysodeikticus DNA (72% GC). |
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