Genetic characterization of brown trout (<Emphasis Type="Italic">Salmo trutta</Emphasis>) populations from the Southern Balkans using mtDNA sequencing and RFLP analysis |
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Authors: | Apostolos P Apostolidis Demetrios Loukovitis Costas S Tsigenopoulos |
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Institution: | (1) Department of Animal Production, Faculty of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece;(2) Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research (HCMR) Crete, Thalassocosmos, 71500 Gournes Pediados, Heraklion, Greece |
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Abstract: | In the present study, a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to survey
variation in a 1450-bp mitochondrial DNA (mtDNA) segment which comprises part of the cytochrome oxidase III (COIII) and ATPase
subunit VI genes in 8 brown trout (Salmo trutta L) populations from the southern Balkans. In addition, a 300 bp fragment at the 5′ end of the control region was sequenced
from representatives of the populations studied providing the opportunity to assign PCR-RFLP haplotypes into major phylogenetic
lineages (i.e. Atlantic, Danubian, marmoratus, Adriatic and Mediterranean). The level of polymorphism found in the 1450 bp segment suggests that this PCR-RFLP assay may
be useful for future diagnostic analyses of mitochondrial DNA in brown trout populations. A reduced within-population genetic
variability but considerable among-population differentiation was observed. The results are in accordance with previous data
on phylogeography of Mediterranean brown trout suggesting that mitochondrial DNA haplotypes are distributed in a mosaic pattern
as a consequence of a complex evolutionary history. The present study shows that brown trout populations from the Southern
Balkans are highly divergent and possess a unique genetic profile that should be taken into account when establishing conservation
management programs.
Handling editor: C. Sturmbauer |
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Keywords: | Southern Balkans Salmo trutta L Mitochondrial DNA PCR-RFLP analysis Control region sequences |
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