质粒治疗肢体动脉闭塞性疾病的安全性分析

肢体动脉血管闭塞性病是一种严重危害人类健康的疾病，许多病人最终要截肢，基因治疗为这类患带来了希望。利用肝细胞生长因子促进血管生成的作用，采用质粒作为载体对大鼠和故事片诉下肢动脉闭塞疾病模型进行治疗，效果良好。为探讨基因治疗的安全性问题，用绿色荧光蛋白报告基因研究质粒在体内的分布和表达，方法简便。通过制作冰冻切片观察绿色荧光蛋白在小鼠体内的表达情况发现局部注射质粒不会造成全身性扩散，表明该方法安全。

Safety evaluation on the treatment of periphery artery disease by plasmid

Periphery artery disease(PAD)is a kind of severe disease that leads to amputation in many patients.Gene therapy brings hope to these patients.We used the angiogenesis effect of hepatocyte growth factor to treat the PAD and found the growth of new vessels.But the safety was an important problem of gene therapy.So we investigated the expression and distribution of green fluorescence protein gene as report gene in mice.The conclusion was that plasmids injected locally did not disperse in the body,so gene therapy of PAD with plasmids was safe.