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| 基于选择性抑制mPGES-1的表达筛选抗炎中药 | |
| 引用本文： | 郭雷,王淑军,龚邦强. 基于选择性抑制mPGES-1的表达筛选抗炎中药[J]. 天然产物研究与开发, 2010, 22(1) |
| 作者姓名： | 郭雷 王淑军 龚邦强 |
| 作者单位： | 1. 江苏省海洋资源开发研究院,淮海工学院;江苏省海洋生物技术重点实验室,淮海工学院,连云港 222005 2. 江苏省海洋资源开发研究院,淮海工学院 3. 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
| 基金项目： | 江苏省海洋资源开发研究院基金，淮海工学院自然科学基金 |
| 摘 要： | 通过建立的选择性膜型前列腺素E2合酶-1(mPGES-1)表达抑制剂的体外细胞筛选模型,对50味中药共236个样品进行筛选。初筛中,筛选中药不同极性提取物对mPGES-1表达的抑制作用,抑制率50%的样品进入复筛;复筛中,检测初筛活性样品对环氧化酶(COX)-1和COX-2表达的抑制作用,抑制率均20%的样品认为是阳性物。结果表明,吲哚美辛对3种酶的抑制活性与文献报道相近;筛选发现泽泻组分1、蒲黄组分2对mPGES-1的表达有可重复的选择性抑制作用,对泽泻组分1、蒲黄组分2进行进一步研究,有望确定其抗炎的有效部位或化合物。 |
| 关 键 词： | 膜型前列腺素E_2合酶-1 炎症 中药 药物筛选 |
Screening of Anti-inflammatory Traditional Chinese Medicine by the Selective Inhibition of mPGES-1 Expression |
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| GUO Lei,WANG Shu-jun,GONG Bang-qiang. Screening of Anti-inflammatory Traditional Chinese Medicine by the Selective Inhibition of mPGES-1 Expression[J]. Natural Product Research and Development, 2010, 22(1) | |
| Authors: | GUO Lei WANG Shu-jun GONG Bang-qiang |
| Affiliation: | GUO Lei1,2,WANG Shu-jun1,GONG Bang-qiang3 1Key Lab of Marine BiotechnoLogy of Jiangsu province,Huaihai Institute of Technology,2Jiangsu Marine Resources R&D Institute,Lianyungang 222005,China,3State Key Laboratory of Bioreactor Engineering,East China University of Science , Technology,Shanghai 200237 |
| Abstract: | To determine selective inhibitors of mPGES-1 expression in vitro from Traditional Chinese Medicine(TCM).In the primary screening,the samples with inhibitory ratio >50% of mPGES-1 expression can enter into the secondary screening,then the samples with inhibitory ratio <20% of COX-1 and COX-2 expression together can be regarded as hits.Total 236 samples from 50 species of TCM were detected.The results showed that indomethacin was shown to be an inhibitor of all 3 enzymes similar with the references.The select... |
| Keywords: | mPGES-1 inflammation traditional Chinese medicine drug screening |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |