Spectrophotometric determination of bromopyruvate by reaction with 2-nitro-5-thiobenzoic acid |
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Authors: | S L Yun C H Suelter |
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Institution: | Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824 USA |
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Abstract: | A spectrophotometric method for determination of bromopyruvate, based on the reaction with 2-nitro-5-thiobenzoic acid, is described. The reaction is complete in 30 min at room temperature in 0.1 m Tris-MES, 1 mm EDTA, pH 8.0. The method is sensitive to at least 1 × 10?5m bromopyruvate. Reagents are stable, easy to prepare, and specific for β-halopyruvate. Bromopyruvate solutions must be prepared fresh daily. Solutions of bromopyruvate at pH 8.0 and 23°C have a half-life of 3 hr. |
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