Immunolocalization of two ligninO-methyltransferases in stems of alfalfa (Medicago sativa L.) |
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Authors: | Rossio Kersey Kentaro Inoue Karel R Schubert and Richard A Dixon |
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Institution: | (1) Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma;(2) Plant Biology Division, The Samuel Roberts Noble Foundation, 2510, Sam Noble Parkway, 73401 Ardmore, OK, USA |
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Abstract: | Summary Caffeic acid 3-O-methyltransferase (COMT) and caffeoyl CoA 3-O-methyltransferase (CCOMT) catalyze parallel reactions that are believed to be involved in the biosynthesis of lignin monomers.
Antisera specific for alfalfa (Medicago sativa L.) COMT or CCOMT were raised against the enzymes expressed inEscherichia coli, and were used for immunolocalization studies in lignifying alfalfa stem tissue. Both COMT and CCOMT were localized to xylem
parenchyma cells, as assessed by light microscopy and immunocytochemistry. Electron microscopy revealed that both enzymes
were located in the cytoplasm of xylem parenchyma cells, and to a lesser extent, in the cytoplasm of phloem cells. There was
no significant difference in the localization pattern of COMT and CCOMT, suggesting that the two enzymes may be part of a
metabolic grid leading to production of lignin monomers in lignifying tissue of mature alfalfa stem internodes. |
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Keywords: | Alfalfa Caffeic acid 3-O-methyltransferase Caffeoyl CoA 3-O-methyltransferase Immunocytochemistry Lignification Xylem parenchyma |
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