Nanoelectrochemistry of cytochrome P450s: Direct electron transfer and electrocatalysis |
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| Authors: | V. V. Shumyantseva T. V. Bulko Yu. O. Rudakov G. P. Kuznetsova N. F. Samenkova A. V. Lisitsa I. I. Karuzina A. I. Archakov |
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| Affiliation: | (1) Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, Moscow, 119121, Russia |
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| Abstract: | Direct electron transfer has been demonstrated between cytochrome P450 2B4 (CYP2B4), P450 1A2 (CYP1A2), sterol 14α-demethylase (CYP51MT) and screen printed graphite electrodes, modified by gold nanoparticles and didodecyldimethyl ammonium bromide (DDAB). The proposed method for preparation of enzymatic nanostructured electrodes may be used for electrodetection of this hemoprotein provided that 2–200 pmol P450 per electrode has been adsorbed. Electron transfer, direct electrochemical reduction and interaction with P450 substrates (oxygen, benzphetamine, lanosterol) and inhibitor ketoconazole were analyzed using cyclic voltammetry (CV), square wave (SWV) or differential pulse (DPV) voltammetry, and amperometry. |
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| Keywords: | cytochrome P450 2B4 P450 1A2 sterol 14α -demethylase from Mycobacterium tuberculosis (CYP51MT, P45014DM) lanosterol ketoconazole screen printed electrodes bioelectrochemistry gold nanoparticles nanostructured electrodes |
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