Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics |
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Authors: | Frank Dolbeare |
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Institution: | (1) Biology and Biotechnology Program, Lawrence Livermore National Laboratory, University of California, 94551-9900 Livermore, CA, USA |
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Abstract: | Summary Bromodeoxyuridine (BrdUrd), a thymidine analogue incorporated into DNA, can be quantified by fluorescent or chromophoric quenching
of dyes bound to DNA or with antibodies to BrdUrd. These technologies have been used since the 1970s as tools for measuring
DNA synthesis in isolated chromosomes and in cells and tissues. This paper is Part I of a three-part comprehensive review
of the literature over the last 20 years (to the end of 1993) describing the histochemical methods for measuring BrdUrd in
cells and tissues. Fixation, denaturation and staining procedures are compared for quantifying BrdUrd for microscopy and flow
cytometry. Non-immunochemical methods related to the quenching of fluorescent DNA stains by BrdUrd are also described. Methods
are described for the comparative assay of cell kinetic parameters by tritiated thymidine and bromodeoxyuridine. The multivariate
BrdUrd/DNA assay of Ts, and Tc, and a comparison of recent methods based on the single biopsy bivariate analysis of Tpot, is presented. Recent developments in the use of double halopyrimidine label to determine kinetic parameters are also reviewed. |
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