| 赤链蛇Sox基因的克隆及序列分析 |
| |
| 引用本文： | 杨超,聂刘旺.赤链蛇Sox基因的克隆及序列分析[J].激光生物学报,2002,11(1):15-18. |
| |
| 作者姓名： | 杨超 聂刘旺 |
| |
| 作者单位： | 安徽师范大学生命科学学院,中国安徽,芜湖,241000 |
| |
| 基金项目： | 安徽省自然科学基金 (NO :97412 0 0 2 ) |
| |
| 摘 要： | 参照人SRY基因HMG-box保守区的序列，设计一对兼并引物，采用PCR技术扩增了赤链蛇的Sox基因，并对扩增产物进行了克隆和测序，结果在雌雄个体中共筛选出三个Sox基因，其中有一个为雌雄共有，显示出性别差异性；三个Sox基因编码的氨基酸序列与人相应SOX3，SOX4，SOX22基因的相似性分别为96%，98%，96%。显示出Sox基因在进化上的高度保守性，本文为赤链蛇的性别决定机制研究提供了分子资料。
|
| 关 键 词： | 赤链蛇 Sox基因 PCR 序列分析 基因克隆 性别决定 |
| 文章编号： | 1007-7146(2002)01-0015-04 |
| 收稿时间： | 2001/9/9 |
| 修稿时间： | 2001年9月9日 |
The Clone and Sequence Analysis of the Sox Genes in Dinodon Rufozonatum |
| |
| YANG Chao,NIE Liu wang.The Clone and Sequence Analysis of the Sox Genes in Dinodon Rufozonatum[J].ACTA Laser Biology Sinica,2002,11(1):15-18. |
| |
| Authors: | YANG Chao NIE Liu wang |
| |
| Abstract: | Using PCR,The Sox gene of Dinodon rufozonatum were amplified with a pair of degenerate primers based on the Human HMG-box gene. By cloning and sequencing the amplification products,we get 3 different Sox genes,among them,only one were amplified from both male and female body. The result indicated that there are different between them. The 3 Sox genes show high homology to Human SOX genes,and the identities to human SOX genes in the DNA sequence and the amino acid sequence are 89%?91%?92% and 96%?98%?96%,respectively. This may be concluded that Sox gene is highly conservative in phylogenetic. This paper provides molecular data for the study of Sex-determining mechanism of Dinodon rufozonatum . |
| |
| Keywords: | Dinodon rufozonatum Sox gene PCR Sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
