Trafficking motifs in the SARS-coronavirus nucleocapsid protein |
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Authors: | You Jae-Hwan Reed Mark L Hiscox Julian A |
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Institution: | Institute of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UK. |
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Abstract: | The severe acute respiratory syndrome-coronavirus nucleocapsid (N) protein is involved in virus replication and modulation of cell processes. In this latter respect control may in part be achieved through the sub-cellular localisation of the protein. N protein predominately localises in the cytoplasm (the site of virus replication and assembly) but also in the nucleus/nucleolus. Using a combination of live-cell and confocal microscopy coupled to mutagenesis we identified a cryptic nucleolar localisation signal in the central part of the N protein. In addition, based on structural comparison to the avian coronavirus N protein, a nuclear export signal was identified in the C-terminal region of the protein. |
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Keywords: | Coronavirus Nucleocapsid protein Nucleolus Nucleolar localisation signal Nuclear export signal Nucleus Confocal microscopy |
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