Dietary fatty acid composition differently influences retinoylation reaction in rat testes mitochondria |
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| Authors: | Erika Cione Valentina Senatore Paola Tucci Anna M. Giudetti Francesco Genchi Gabriele V. Gnoni Giuseppe Genchi |
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| Affiliation: | (1) Department of Pharmaco-Biology, Laboratory of Biochemistry, University of Calabria, Edificio Polifunzionale, 87036 Rende (CS), Italy;(2) Department of Biological and Environmental Sciences and Technologies,Laboratory of Biochemistry , University of Lecce, Via Provinciale Lecce-Monteroni, 73100 Lecce, Italy;(3) Department of Pharmaco-Biology, Laboratory of Biochemistry, University of Bari, via Orabona 4, 70125 Bari, Italy;(4) Dipartimento Farmaco-Biologico, Università della Calabria, 87036 Rende (CS), Italy |
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| Abstract: | All-trans-retinoic acid (atRA) is incorporated covalently into proteins of rat testes mitochondria. In this study, the effect of three diets with different fatty acid composition on the retinoylation of proteins of rat testes mitochondria has been investigated. Different groups of rats were fed on a basal diet supplemented with 15% of either coconut oil (CO), olive oil (OO) or fish oil (FO). We found that, when compared with CO, the binding of retinoic acid was decreased in FO- and OO-fed rats. Mitochondrial phospholipids composition was differently influenced by dietary treatments; minor changes were observed in fatty acid composition of phospholipids. Few differences were observed in the Arrhenius plots among the three groups of rats. Kinetic analysis revealed a decrease in the V max value in FO- and OO- as compared with CO-fed rats. No difference among the three groups were observed in the K M value. The retinoylation reaction was inhibited by 13-cis-RA and 9-cis-RA. |
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| Keywords: | All-trans-retinoic acid Fatty acids Mitochondria Phospholipids Retinoylation |
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