Protection against reactive oxygen species by NAD(P)H: quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Decreased hepatic low-level chemiluminescence during quinone redox cycling |
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Authors: | H Wefers T Komai P Talalay H Sies |
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Affiliation: | 1. Department of Genetics and Microbiology, Section of Crystallography, University of Pavia, Via Taramelli 16, I-27100 Pavia, Italy;2. Department of Biomedical Science and Technology, University of Milano, Via Olgettina 60, I-20132 Milano, Italy |
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Abstract: | Menadione elicits low-level chemiluminescence (lambda greater than 620 nm) associated with redox cycling of the quinone in mouse hepatic postmitochondrial fractions. This photoemission is suppressed when the animals are fed a diet containing the anticarcinogenic antioxidant, 2[3]-(tert-butyl)-4-hydroxyanisole (BHA), which leads to a 13-fold increase in NAD(P)H: quinone reductase (EC 1.6.99.2). Inhibition of the enzyme by dicoumarol completely abolishes the protective effect of BHA treatment and leads to higher chemiluminescence, reaching similar photoemission for BHA-treated and control animals. These findings indicate that the two-electron reduction promoted by quinone reductase prevents redox cycling and that BHA protects against reactive oxygen species by elevating the activity of this enzyme. |
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Keywords: | Ferritin Isoferritin Protein crystal Iron protein Iron metabolism |
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