The metabolism of l-serylglycine O[35S]-sulphate in the rat |
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Authors: | N Tudball Y Noda and K S Dodgson |
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Institution: | Department of Biochemistry, University College, St Andrew''s Place, Cardiff |
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Abstract: | 1. The preparation of potassium l-serylglycine O-sulphate and the corresponding 35S-labelled ester is described. 2. Intraperitoneal injection of potassium l-serylglycine O35S]-sulphate to rats results in about 75% of the radioactivity of the dose appearing in the urine within 48hr. Almost 72% of the radioactivity recovered in the urine was in the form of inorganic 35S]sulphate. 3. Analysis of urines by paper chromatography showed the presence of unchanged l-serylglycine O35S]-sulphate and several other unidentified 35S-labelled materials. 4. It has been established that micro-organisms of the gastrointestinal tract do not play any significant role in the production of inorganic 35S]sulphate from the injected ester. 5. l-Serylglycine O-sulphate was hydrolysed by crude dipeptidase preparations from rat kidney and intestine to yield l-serine O-sulphate and glycine as the sole products. |
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