Evaluation of MCM-2 expression in TMA cervical specimens |
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Authors: | Nicol Alcina F Lapa e Silva José R Cunha Cynthia B Amaro-Filho Sergio M Oliveira Nathalia Grinsztejn Beatriz Friedman Ruth Khalili Khalil Ruth Russomano Fabio Pires Andrea Golub Jonathan E Nuovo Gerard J |
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Institution: | Laboratory Interdisciplinary of Medical Research, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil. nicol@ioc.fiocruz.br |
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Abstract: | BackgroundMinichromosome maintenance proteins (MCM) are highly expressed in actively replicating cells. The need for biological markers for cervical carcinoma and its precursor lesions is emerging. Our main aim was to determine the immunohistochemical expression of MCM-2 in HIV-positive and -negative dysplastic cervical specimens.MethodsImmunohistochemical analysis of MCM-2 was performed in a total of 352 cervical TMA specimens of normal control, low-grade CIN, high-grade CIN and invasive tumor. 38 specimens were from HIV-positive women. A receiver operating characteristic (ROC) curve was constructed to determine the best cutoff to diagnose high-grade CIN and invasive cervical cancer.ResultsIn the progression from normal epithelium to high-grade CIN and invasive tumor we found significant differences in the MCM-2 expression (p<0.05). Based on the ROC curve of 80% with an area under the curve (AUC) of 0.78, expression of MCM-2 to diagnose high-grade CIN and invasive tumor resulted in sensitivity of 81%, specificity of 66%, a positive predictive value (PPV) of 86% and a negative predictive value (NPV) of 57%. HIV-positive cervices revealed a decreasing expression of MCM-2 in both LGCIN and HGCIN compared with HIV-negative specimens (p<0.0001).ConclusionsThe present study suggests that immunohistochemical MCM-2 may not be a promising biomarker for diagnosing high-grade CIN and invasive cancer. |
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