In vitro modification of Candida albicans invasiveness |
| |
Authors: | Silvia E. Fontenla de Petrino M. Eugenia B. B. de Jorrat Angel Sirena Juan Carlos Valdez Oscar Mesón |
| |
Affiliation: | (1) Instituto de Microbiología Dr. Luis C. Verna Facultad de Bioqui'mica, Universidad Nacional de Tucumán, Ayacucho 491, 4000 San Miguel de Tucumán, República Argentina |
| |
Abstract: | Candida albicans produces germ-tubes (GT) when it is incubated in animal or human serum. This dimorphism is responsible for its invasive ability.The purpose of the present paper is (1) to evaluate the ability of rat peritoneal macrophages to inhibit GT production of ingested Candida albicans, obtained from immunized rats and then activated in vitro with Candida-induced lymphokines; (2) to determinate any possible alteration of phagocytic and candidacidal activities.The phagocytes were obtained from rats immunized with viable C. albicans. Some of them were exposed to Candida-induced lymphokines in order to activate the macrophages in vitro. The monolayers of activated, immune and normal macrophages were infected with a C. albicans suspension during 4 hr.Activated macrophages presented not only the highest phagocytic and candidacidal activities but a noticeable inhibition of GT formation and incremented candidacidal activity. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |