杜氏盐藻（Dunaliella salina）基因工程的研究现状及应用前景

杜氏盐藻（简称盐藻）作为新型的生物反应器，成为藻类基因工程的研究热点之一。利用基因工程手段对盐藻进行遗传改造以生产外源物质是目前研究的重要领域。本文从盐藻相关基因的克隆、cDNA文库的建立、基因组文库的构建、筛选标记的确立和外源基因的表达等五个方面全面概述了国内外盐藻基因工程的研究进展，特别是对最新的研究结果进行了综述，并对基因工程技术在盐藻深入研究中的应用做了前景展望。

Research Status and Application Prospect of Gene Engineering

Dunaliella salina(D.salina)as a new novel bioreactor has been a research hotspot of algal gene engineering.Genetic reconstruction of D.salina via gene engineering for production of foreign materials has become an important research field.A comprehensive summary of the research status of D.salina genetic engineering,especially in the current research development,including the related gene cloning;construction of cDNA library and genomic library;establishment of selectable marker and foreign gene expression were given,and a prospect of the future application of gene engineering in the research of D.salina were also given.