| How does radiation damage in protein crystals depend on X-ray dose? |
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| 作者姓名： | Sliz P Harrison SC Rosenbaum G |
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| 收稿时间： | 30 July 2002 |
How does Radiation Damage in Protein Crystals Depend on X-Ray Dose? |
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| Sliz P,Harrison SC,Rosenbaum G.How does Radiation Damage in Protein Crystals Depend on X-Ray Dose?[J].Structure,2003,11(1):13-19. |
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| Authors: | Sliz Piotr Harrison Stephen C Rosenbaum Gerd |
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| Institution: | Department of Biological Chemistry and Molecular Pharmacology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA. |
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| Abstract: | Is radiation damage to cryopreserved protein crystals strictly proportional to accumulated dose at the high-flux density of beams from undulators at third-generation synchrotron sources? The answer is “yes,” for overall damage to several different kinds of protein crystals at flux densities up to 1015 ph/sec/mm2 (APS beamline 19-ID). We find that, at 12 keV (1 Å wavelength), about ten absorbed photons are sufficient to “kill” a unit cell. As this corresponds to about one elastically scattered photon, each unit cell can contribute only about one photon to total Bragg diffraction. The smallest crystal that can yield a full data set to 3.5 Å resolution has a diameter of about 20 μm (100 Å unit cell). |
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