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Microscale isoelectric focusing studies of mouse and human hypoxanthine-guanine phosphoribosyl transferases
Authors:Kenneth L Roy  Frank H Ruddle
Institution:(1) Department of Biology, Yale University, New Haven, Connecticut;(2) Present address: Department of Microbiology, University of Alberta, Edmonton, Alberta, Canada
Abstract:A microscale isoelectric focusing technique has been developed and used to study hypoxanthine-guanine phosphoribosyl transferase (HGPRT; E.C. 2.4.2.8, inosinate-guanylate:pyrophosphate phosphoribosyl transferase) activities in mouse and human cell lines. The enzymes of both mouse and human origin are shown to exhibit considerable heterogeneity, but each type has a unique range of isoelectric pH. The enzyme of a mouse × human hybrid cell line, derived by fusion of HGPRT parental cells, gives a homogeneous peak of activity, unlike the wild-type enzyme of either parent. The possibility is suggested that this enzyme activity is due to intra-allelic complementation.Centennial Fellow of the Medical Research Council of Canada, 1967–1970.
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