两种水溶性抗菌活性物质的分离提取

对水溶性、不解离的极性物质分离时，一般采用吸附层析和凝胶层析等途径。实验通过硅胶柱层析、葡聚糖凝胶柱层析以及硅胶ＧＦ２５４制备型薄板层析，从发酵液样品中分离出两种有抗菌活性的纯物质。薄层层析的展开剂为二氯甲烷－四氢呋喃－甲醇－水（２５：３０：２），分离出的组分中Ｒｆ＝０．７和Ｒｆ＝０．８两种物质有抗菌活性。硅胶柱层析洗脱过程为梯度洗脱，先用１５０ｍｌ上述展开剂洗脱，再用二氯甲烷－甲醇（２０：８０）

Isolation and Extraction of Two Hydrophilic Antibiotic Substances

Adsorption chromatography and gel filtration were usually used in isolating hydrophilic, non-dissociated and polar substance. In the test, two antibiotic substances were isolated by chromatography, such as silica gel column chromatography, gel filtration chromatography and silica gel (GF254) thin-layer chromatography. The developer of TLG was methylene dichloride-tetrahydro-furan-methyl alcohol-water (25:30:43:2), the retardation factors of the two antibiotics were 0.7 and 0.8. The silica gel column chromatography was used for gradient elution and the first 150ml eluent was the same as above developer; the next 50ml eluent was methylene dichloride-methyl alcohol (20:80); the last 50ml eluent was pure methyl alcohol. Both the antibiotic substances verified by HPLC were pure.