新加良附方对移植性小鼠肝癌抑制率及其凋亡影响研究

目的:观察新加良附方对移植性小鼠肝癌(H22)生长抑制作用及其对肿瘤组织中Bcl-2和Bax表达影响。方法:建立移植型H22动物模型,并将动物模型随机分模型对照、环磷酰胺(CTX)与新加良附方大、中、小剂量5组。新加良附大、中、小剂量组给药量分别为10g/kg、5g/kg和2.5g/kg;CTX组给药计量为17mg/kg;模型组给予等量无菌生理盐水。连续给药、给水12天处死模型小鼠分离肿瘤,检测肿瘤大小、称重计算肿瘤抑制率,并将肿瘤组织切片,免疫组化法检测Bcl-2和Bax基因。结果:新加良附大剂量组肿瘤抑制率为48.5%,与模型对照组比较,有统计学意义(P<0.01);新加良附大、中剂量可降低肿瘤组织中Bcl-2蛋白基因表达,升高Bax蛋白基因表达,与模型组比较,有显著性差异(P<0.01,P<0.05)。结论:新加良附方可抑制H22瘤体生长,且下调Bcl-2蛋白基因表达,上调Bax基因,提示新加良附方在抗肿瘤方面具有进一步深入研究价值。

Effect of Xinjia Liangfu Recipe on Tumor Inhibition Rate and Apoptosis of Transplanted Hepatocarcinoma in Mice

Objective:To observe the effect of Xinjia Liangfu recipe on tumor growth of transplanted hepato-carcinaoma in mice and expressions of Bcl-2 and Bax proteins in tumor tissues.Methods:50 healthy Kunming mice,, transplanted by H22 hepatocarcinoma cells, were divided into 5 groups, including model group, cyclophosphamide group and three groups of Xinjia Liangfu recipe in high dose(10g/kg), medium dose(5g/kg) and low dose(2.5g/kg).The dose of cyclophosphamide group was 17mg/kg, the model group were treat-ed with the same volume germfree saline.The mice were killed after twelve days of the drugs and water treatment and tumor tissues were separated.The size and weight of tumor tissues were measured and the tumor inhibition rate was calculated.And expressions of Bcl-2 and Bax were detected by using immunohistochemical method.Result:The tumor inhibition rate of Xinjia Liangfu recipe group in high dose was 48.5% and was higher obviously than that in model group(P<0.01).Compared to the model group, expression of Bcl-2 protein increased and the expres-sion of Bax protein decreased in the groups of Xinjia Liangfu recipe in high dose and medium dose(P<0.01, P<0.05).Conclusion:Xinjia Liangfu recipe could inhibit the growth of tumor of H22, reduce the expression of Bcl-2 protein and raise the expression of Bax,which is worth further studying in antitumor.