灵芝原生质体制备、再生及融合的研究

报道不同菌龄、酶液浓度、稳渗剂对灵芝原生质体产率及不同种类、不同浓度的稳渗剂对原生质体再生的影响。结果表明，诱变后具抗药性标记的“中国红灵芝”菌种（mH1）原生质体制备时菌丝最适菌龄为48小时，酶液浓度为3％，稳渗剂以0．6mol／L蔗糖为佳；诱变后具抗药性标记的“韩国红灵芝”（mK1）原生质体制备时菌丝最适菌龄为40小时，酶液浓度为1％，稳渗剂为0.4mol／L甘露醇时原生质体产率最高。mH1，mK1原生质体均以蔗糖作稳渗剂再生率较高，其最佳浓度为0．6mol／L。在该条件下mH1原生质体再生率为3．20×10-2，mK1为5．40×10-2。在此基础上，以30％的PEG作为融合诱导剂进行灵芝原生质体融合，并获得了融合子，融合率为140×10-2。

PREPARATION,REGENERATION AND FUSION OF THE PROTOPLAST OF GANODERMA LUCIDUM

This paper studied the output and regeneration and fusion of theprotoplastS of strains mH1 and mK1 of Ganoderma lucidum. The results showed thatthe output of the protoplastS was different under different age of hyphae, enzymeconsistency and stabliser. For strain mH1, its optimal age of hyphe, enzymeconsistency and stabliser was 48hr, 3% and 0.6mol / L sucrose respechvely, the strainmK1 was 40hr, 1% and 0.4mol / L mannitol. The frequency of regenerahon of thetwo strains were the best and 3.20 X 10-2 (strains mH1) and 5.40 X 10-2 (strain mKI)sep~ly when the stabliser was 0.6mol / L sucrose. Fusion experimentS betWeen theabove tWo strains were done. The frequency of fusion, after treating with 30% PEG,was 1 .40 X 10 -2.