Repartition of ATP, ADP and PO4 in isolated hepatocytes from fed and fasted rats |
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Authors: | A Lavoinne |
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Institution: | Laboratoire de Biochimie Médicale, U.E.R. Médecine-Pharmacie de Rouen, Avenue de l''Université, B.P. 97, 76800 Saint-Etienne-du-Rouvray, France |
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Abstract: | 1. The digitonin fractionation procedure Zuurendonk, P. F. and Tager, J. M. (1974) Biochim. Biophys. Acta, 333, 393-399] was used to determine the repartition of adenine nucleotides and inorganic phosphate in isolated hepatocytes from fed and fasted rats. 2. This repartition is not significantly modified in the presence of pyruvate or alanine or lactate + pyruvate for isolated hepatocytes from fasted rats. 3. In isolated hepatocytes from fasted rats, the mitochondrial ATP/ADP X PO4 ratio is two-fold lower than in isolated hepatocytes from fed rats. 4. The cytosolic ATP/ADP X PO4 ratio depends on the nutritional state and (or) on the added substrate for neoglucogenesis. |
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Keywords: | mitochondries cytosol nucléotides adényliques état de phosphorylation mitochondria cytosol adenine nucleotides phosphorylation state |
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