Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers |
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Authors: | Fox Jeffrey D Routzahn Karen M Bucher Matthew H Waugh David S |
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Institution: | Protein Engineering Section, Macromolecular Crystallography Laboratory, National Cancer Institute at Frederick, P.O. Box B, Frederick, MD 21702-1201, USA. |
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Abstract: | In the biosynthesis of the C7-cyclitol moiety, valienol, of the -glucosidase inhibitor acarbose in Actinoplanes sp. SE50/110 various cyclitol phosphates, such as 1-epi-valienol-7-phosphate, are postulated precursors. In the cell extracts of Actinoplanes SE50/110 we found a new kinase activity which specifically phosphorylates 1-epi-valienol; other C7-cyclitol analogs were only weakly or not phosphorylated. The purified product of the kinase reaction turned out to be 1-epi-valienol-7-phosphate in analyses by nuclear magnetic resonance spectroscopy. The enzyme seems not to be encoded by an acb gene and, therefore, plays a role in a salvage pathway rather than directly in the de novo biosynthesis of acarbose. |
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Keywords: | Acarbose Biosynthesis 1-epi-Valienol C7-cyclitol 7-kinase Actinoplanes sp |
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