Characterisation of an autonomously replicating sequence from the fission yeast Schizosaccharomyces pombe |
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| Authors: | Leland H. Johnston and David G. Barker |
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| Affiliation: | (1) Laboratory of Cell Propagation, National Institute for Medical Research, Mill Hill, NW7 AA London, UK;(2) Present address: Laboratoire de Biologie des Relations Plantesmicroorganisms, Chemin de Borde Rouge, Auzeville, F-31320 Castanet-Tolosan, France |
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| Abstract: | Summary A DNA sequence has been isolated from Schizosaccharomyces pombe which promotes high frequency transformation of plasmids in the same organism. It is closely linked to the DNA ligase gene CDC17 and has therefore been named ARS17 although in structure it differs substantially from ARS elements in Saccharomyces cerevisiae. ARS17 spans some 1.8 kb of DNA and deletion of any part of this region affects activity. Moreover, there does not appear to be any short sequence which is, by itself, sufficient for high frequency transformation. ARS17 lies between and partly overlaps two divergently transcribed genes and it is extremely AT rich. It lacks the consensus sequence found in S. cerevisiae ARSs and it has no ARS activity in S. cerevisiae. |
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| Keywords: | Origin of replication ARS Yeast |
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