Nucleotide sequence of the rci gene encoding shufflon-specific DNA recombinase in the IncI1 plasmid R64: homology to the site-specific recombinases of integrase family |
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Authors: | Akihiro Kubo Atsuko Kusukawa and Teruya Komano |
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Institution: | (1) Department of Biology, Tokyo Metropolitan University, Fukazawa, Setagaya-ku, 158 Tokyo, Japan;(2) Present address: Environmental Biology Division, National Institute for Environmental Studies, Yatabe-machi, 305 Tsukuba, Ibaraki, Japan;(3) Present address: Wako Research Center, Honda R&D Co. Ltd., 351-01 Wako, Saitama, Japan |
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Abstract: | Summary Shufflon is a novel type of DNA rearrangement in which four DNA segments are flanked by seven 19-bp repeat sequences. The site-specific recombination between any inverted repeats results in an inversion of the DNA segment(s) either independently or in groups. The recombination is mediated by a gene designated rci. We have determined the nucleotide sequence of the rci gene and found that it encodes a basic protein with 384 amino acid residues. The rci gene was fused with lacZ and its gene product was identified by Western blot analysis. The Rci protein shows regional homologies to the site-specific recombinases encoded by the bacteriophage genomes, including those of , 80, P22, P2, 186, P4 and P1. |
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Keywords: | DNA rearrangement R64 ric gene Recombination Shufflon |
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