Isolation and characterisation of a bovine cDNA encoding eukaryotic initiation factor 2 alpha |
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| Authors: | S R Green J Fullekrug K Sauer M F Tuite |
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| Institution: | Biological Laboratory, University of Kent, Canterbury, U.K. |
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| Abstract: | Two cDNA clones have been isolated, from a bovine lymphosarcoma library, that encode the alpha-subunit of eukaryotic initiation factor 2 (eIF-2 alpha). The predicted 315 amino acid sequence showed more than 99% amino acid identity with rat and human eIF-2 alpha. Galactose-regulated expression of a full length bovine eIF-2 alpha cDNA in yeast resulted in the synthesis of a polypeptide of the predicted molecular mass (36 kDa). Furthermore, the expressed polypeptide cross-reacted with an antibody raised against rabbit eIF-2 alpha confirming the identity of the cDNA. |
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