The effect of lead ions on the energy metabolism of human erythrocytes in vitro |
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Authors: | Baranowska-Bosiacka Irena Hlynczak Alina Joanna |
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Affiliation: | Department of Biochemistry, University of Szczecin, 3a Felczaka st, 71-412 Szczecin, Poland. ika@univ.szczecin.pl |
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Abstract: | The aim of this work was to evaluate the influence of chronic exposure to lead ions on the parameters of energetic status of human erythrocytes in vitro. Umbilical cord erythrocytes were incubated with lead acetate at final lead ion concentrations ranging from 10 to 200 microg/dl. ATP, ADP, AMP, adenosine, GTP, GDP, GMP, guanosine, IMP, inosine, hypoxanthine, NAD and NADP concentrations in erythrocytes were determined using HPLC. Scanning electron micrographs of erythrocytes were taken. The mean concentrations of ATP, GTP, NAD and NADP, and mean values of adenylate energy charge (AEC) and GEC in cells incubated at the presence of lead ions were significantly lower after 20 h of incubation. Concentrations of purine degradation products (Ado, Guo, Ino) and Hyp were significantly higher. It is suggested that lead ions affect the energy metabolism of erythrocytes. Morphological changes in erythrocytes correspond to the increase of lead ions in the incubation mixture and to the decrease of ATP concentration in erythrocytes. A decrease in NAD and ATP concentration in erythrocytes could be a sensitive indicator of energy process disturbance, useful in monitoring in case of chronic lead exposure. |
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Keywords: | Erythrocytes Energy charge Energy metabolism Lead ions HPLC technique Purine nucleotide Ado, adenosine AEC(GEC), adenylate (guanylate) energy charge (CB), umbilical cord blood G6PD, glucose-6-phosphate dehydrogenase Guo, guanosine HK, hexokinase HPLC, high performance liquid chromatography Hyp, hypoxantine TAN(TGN), total adenine (guanine) nucleotides Pb-B, lead in whole blood PDHc, pyruvate dehydrogenase PFK I, phosphofructokinase I PK, pyruvate kinase 6PGD, 6-phosphogluconate dehydrogenase ROS, reactive oxygen species. |
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