Purification and characterization of glycosylated and non-glycosylated forms of prolactin from the dromedary (Camelus dromedarius) |
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Authors: | N Martinat A Anouassi J C Huet J C Pernollet Y Combarnous |
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Affiliation: | Station de Physiologie de la Reproduction, INRA and URA CNRS 1291, Nouzilly, France. |
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Abstract: | 1. Camel prolactin (camPRL) was isolated from the insoluble residue left after extraction of the gonadotropins FSH and LH from a single batch of one-humped camels (Camelus dromedarius) pituitaries. 2. Several isoforms of camPRL were isolated and characterized. 3. Glycosylated and non-glycosylated forms of camPRL were separated by affinity chromatography on Con-A Sepharose. 4. The glycosylated form represented more than 20% of total camPRL and was found to be only 10-20% as active as the non-glycosylated form in its ability to bind to rabbit mammary gland PRL receptors and to stimulate the proliferation of Nb2 cells. |
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