Oligonucleotide Primers for PCR Amplification of Coelomate Introns |
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Authors: | Simon N Jarman Robert D Ward Nicholas G Elliott |
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Institution: | (1) CSIRO Marine Research, GPO Box 1538, Hobart, TAS 7001, Australia, AU |
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Abstract: | Abstract Seven novel oligonucleotide primer pairs for polymerase chain reaction amplification of introns from nuclear genes
in coelomates were designed and tested. Each pair bound to adjacent exons that are separated by a single intron in most coelomate
species. The primer sets amplified introns in species as widely separated by the course of evolution as oysters (Mollusca:
Protostoma) and salmon (Chordata: Deuterostoma). Each primer set was tested on a further 6 coelomate species and found to
amplify introns in most cases. These primer sets may therefore be useful tools for developing nuclear DNA markers in diverse
coelomate species for studies of population genetics, phylogenetics, or genome mapping. |
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