| 抗胶质瘤细胞单克隆抗体SZ-38及其识别抗原的生化特征 |
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| 引用本文： | 杨伟廉,王尧,韦永新,阮长耿,杜子威. 抗胶质瘤细胞单克隆抗体SZ-38及其识别抗原的生化特征[J]. 中国生物化学与分子生物学报, 1988, 4(6): 531-537 |
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| 作者姓名： | 杨伟廉 王尧 韦永新 阮长耿 杜子威 |
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| 作者单位： | 苏州医学院脑神经研究室，苏州医学院脑神经研究室，苏州医学院脑神经研究室，苏州医学院血栓止血研究室，苏州医学院脑神经研究室 苏州，苏州，苏州，苏州 |
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| 摘 要： | 本文以人脑胶质瘤细胞系SHG-44为抗原,利用杂交瘤技术建立了一株恒定地分泌抗胶质瘤细胞单克隆抗体(McAb)的杂交瘤细胞株SZ-38。McAb SZ-38与9/10胶质瘤细胞系发生强结合反应。而与淋巴细胞、ABO型红细胞等所有正常血液细胞及绝大多数被检测肿瘤细胞系无反应。经免疫转移电泳及免疫沉淀法鉴定,该McAb识别抗原为胶质瘤细胞膜上Mw47,000糖蛋白。应用SPA-Sepharose 4B提纯MeAb SZ-38,再把纯化抗体交联于Sepharose 4B,以McAb亲和层析法提取SZ-38抗原,经SDS-PAGE证实其有较高的纯度。
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| 关 键 词： | 单克隆抗体 胶质瘤相关抗原 胶质瘤 |
| 收稿时间： | 1988-12-20 |
BIOCHEMICAL CHARACTERISTICS OF ANTI-GLIOMA MONOCLONAL ANTIBODY SZ-38 AND ITS RECOGNIZED ANTIGEN |
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| Yan,Wei-Han Wan,Yao Du,Zi-wei. BIOCHEMICAL CHARACTERISTICS OF ANTI-GLIOMA MONOCLONAL ANTIBODY SZ-38 AND ITS RECOGNIZED ANTIGEN[J]. Chinese Journal of Biochemistry and Molecular Biology, 1988, 4(6): 531-537 |
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| Authors: | Yan Wei-Han Wan Yao Du Zi-wei |
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| Affiliation: | Brain Research Laboratory.Suzhou Medical College.Suzhou |
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| Abstract: | One hybridoma line SZ-38 secreting monoclonal antibody against the human glioma cell had been established by fusion between NS-1 myeloma cells and spleen cells from a mouse immunized with human glioma cell line SHG-44. McAb SZ-38 strongly bound to 9/10 glioma cell lines and showed no crossreaction with normal blood cells and 12 different human tissues The antigen recognized by McAb SZ-38 was analyzed by Western blotting and immunoprecipitation. It was a glycoprotein present on the membrane of glioma cells with a Mw of 47,000. The SZ-38 antigen was successfully purified by IgG SZ-38-Sepharose 4B affinity chromatography. |
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| Keywords: | Monoclonal antibody Glioma associated antigen Glioma |
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