Plasmid selection in <Emphasis Type="Italic">Escherichia coli</Emphasis> using an endogenous essential gene marker |
| |
Authors: | Shan Goh Liam Good |
| |
Institution: | (1) Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, SE-17177, Sweden;(2) Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, AL9 7TF, UK |
| |
Abstract: | Background Antibiotic resistance genes are widely used for selection of recombinant bacteria, but their use risks contributing to the
spread of antibiotic resistance. In particular, the practice is inappropriate for some intrinsically resistant bacteria and
in vaccine production, and costly for industrial scale production. Non-antibiotic systems are available, but require mutant
host strains, defined media or expensive reagents. An unexplored concept is over-expression of a host essential gene to enable
selection in the presence of a chemical inhibitor of the gene product. To test this idea in E. coli, we used the growth essential target gene fabI as the plasmid-borne marker and the biocide triclosan as the selective agent. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|