Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assay |
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| Authors: | Lisa J Haney James G Coors Aaron J Lorenz DRaj Raman Robert P Anex MPaul Scott |
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| Institution: | (1) Syngenta Seeds Inc, Bloomington, IL, USA;(2) Department of Agronomy, University of Wisconsin-Madison, Madison, WI, USA;(3) Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA, USA;(4) Corn Insects and Crop Genetics Research Unit, ARS, USDA, USA |
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| Abstract: | Background The availability and low cost of lignocellulosic biomass has caused tremendous interest in the bioconversion of this feedstock
into liquid fuels. One measure of the economic viability of the bioconversion process is the ease with which a particular
feedstock is hydrolyzed and fermented. Because monitoring the analytes in hydrolysis and fermentation experiments is time
consuming, the objective of this study was to develop a rapid fluorescence-based method to monitor sugar production during
biomass hydrolysis, and to demonstrate its application in monitoring corn stover hydrolysis. |
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