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CD2AP contributes to cell migration and adhesion in cultured gastric epithelium
Authors:Mustonen Harri  Lepistö Anna  Lehtonen Sanna  Lehtonen Eero  Puolakkainen Pauli  Kivilaakso Eero
Institution:Department of Surgery, Helsinki University Central Hospital, Finland. harri.mustonen@helsinki.fi
Abstract:The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell contacts and F-actin formed a branched network and adhesion belts. In migrating cells, CD2AP appeared as thread-like accumulations in the leading edges, colocalizing with F-actin and occasionally with E-cadherin. Intracellular injection of anti-CD2AP significantly retarded the migration speed of the cells suggesting a crucial role for CD2AP in mucosal cell migration, possibly as a scaffolding protein between cell membrane proteins and actin cytoskeleton. Co-immunoprecipitation assays revealed that CD2AP and E-cadherin are in a complex in HGF stimulated cells. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium.
Keywords:Cell adhesion  Co-immunoprecipitation  Cytoskeleton  Protein interaction
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