| 改善大分子质粒DNA重组效率的新方法 |
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| 引用本文： | 谭德勇,邓双胜,孟玲,昝瑞光.改善大分子质粒DNA重组效率的新方法[J].生物化学与生物物理进展,1997,24(3):281-283. |
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| 作者姓名： | 谭德勇 邓双胜 孟玲 昝瑞光 |
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| 作者单位： | 云南大学生物系，昆明 650091;云南大学生物系，昆明 650091;云南大学生物系，昆明 650091;云南大学生物系，昆明 650091 |
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| 基金项目： | 云南省教委基金和云南省科委应用基础研究基金资助项目. |
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| 摘 要： | 采用两次连接法对一个质粒载体为7.65 kb，插入子为1.47 kb的片段进行重组连接，使连接效率大大提高.此方法对于大分子的质粒DNA重组是非常有效的.
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| 关 键 词： | 质粒DNA，重组效率，二次连接法 |
| 收稿时间： | 4/2/1996 12:00:00 AM |
| 修稿时间： | 8/2/1996 12:00:00 AM |
Improving the Recombination Efficiency of Larger Plasmid DNA by Two-ligation |
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| TAN Deyong,DENG Shuangsheng,MENG Ling and ZAN Ruiguang.Improving the Recombination Efficiency of Larger Plasmid DNA by Two-ligation[J].Progress In Biochemistry and Biophysics,1997,24(3):281-283. |
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| Authors: | TAN Deyong DENG Shuangsheng MENG Ling and ZAN Ruiguang |
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| Institution: | Department of Biology, Yunnan University, Kunming 650091, China;Department of Biology, Yunnan University, Kunming 650091, China;Department of Biology, Yunnan University, Kunming 650091, China;Department of Biology, Yunnan University, Kunming 650091, China |
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| Abstract: | A method of improving the recombination efficiency of larger plasmid DNA is introduced. The major manipulation of this method is that the ligation reaction between insertor and vector is separated from cycle reaction of ligation, and they carry out in different reaction condition. Using this method, the efficiency of the recombination between 7.65 kb vector and 1.47 kb insertor is improved obviously. It is a good method for recombination of larger plasmid DNA. |
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| Keywords: | plasmid DNA recombinatin efficiency two-ligation |
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