Gamma-irradiated plant callus tissue: Cytokinins from transfer ribonucleic acid |
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| Authors: | K. N. Pandey T. R. Kemp P. S. Sabharwal R. A. Andersen |
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| Affiliation: | (1) Thomas Hunt Morgan School of Biological Sciences, University of Kentucky, 40546 Lexington, Kentucky;(2) College of Agriculture, University of Kentucky, 40546 Lexington, Kentucky;(3) Horticulture Department, College of Agriculture, University of Kentucky, 40546 Lexington, Kentucky |
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| Abstract: | Summary Callus tissue ofHaworthia mirabilis Haw. was irradiated with60Co gamma rays. tRNA was isolated, hydrolyzed enzymatically, and cytokinin-active ribonucleosides were separated by Sephadex LH-20 column chromatography and assayed with the tobaccocallus cytokinin bioassay. Three cytokinins were detected in tRNA from irradiated tissue, two of which chromatographed with zeatin riboside and N6-(Δ2-isopentenyl)adenosine. The third cytokinin-active ribonucleoside was retained longer than the above compounds on the Sephadex column and may be 2-methylthio-N6-(Δ2-isopentenyl)adenosine. Two cytokinins were detected in tRNA from nonirradiated tissue—those chromatographed with zeatin riboside and N6-(Δ2-isopentenyl)adenosine. Relationships between cytokinins from tRNA and free cytokinins found in tissue earlier are discussed. This is paper 78-10-124 of the Kentucky Agricultural Experiment Staton and is published with approval of the Director. |
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| Keywords: | cytokinins tRNA 60Co gamma rays callus Haworthia mirabilitis |
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