首页 | 本学科首页   官方微博 | 高级检索  
     


Use of methylation filtration and C(0)t fractionation for analysis of genome composition and comparative genomics in bread wheat
Authors:Bandopadhyay Rajib  Rustgi Sachin  Chaudhuri Rajat Kanti  Khurana Paramjit  Khurana Jitendra Paul  Tyagi Akhilesh Kumar  Balyan Harindra Singh  Houben Andreas  Gupta Pushpendra Kumar
Affiliation:Department of Genetics & Plant Breeding, Ch. Charan Singh University, Meerut, India.
Abstract:We investigated the compositional and structural differences in sequences derived from different fractions of wheat genomic DNA obtained using methylation filtration and C(0)t fractionation. Comparative analysis of these sequences revealed large compositional and structural variations in terms of GC content, different structural elements including repeat sequences (e.g., transposable elements and simple sequence repeats), protein coding genes, and non-coding RNA genes. A correlation between methylation status [determined on the basis of selective inclusion/exclusion in methylation-filtered (MF) library] of different repeat elements and expression level was observed. The expression levels were determined by comparing MF sequences with expressed sequence tags (ESTs) available in the public domain. Only a limited overlap among MF, high C(0)t (HC), and ESTs was observed, suggesting that these sequences may largely either represent the low-copy non-transcribed sequences or include genes with low expression levels. Thus, these results indicated a need to study MF and HC sequences along with ESTs to fully appreciate complexity of wheat gene space.
Keywords:Methylation--filtration  Cot fractionation  Transposable elements  Gene-rich regions
本文献已被 维普 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号