A Simple Method for Collecting and Mounting Ribboned Serial Sections of Epoxy Embedded Specimens |
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Authors: | Suzanne M. Royer |
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Affiliation: | a Department of Molecular, Cellular and Developmental Biology, Campus Box 347, University of Colorado, Boulder, Colorado |
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Abstract: | A simple and rapid method of handling ribboned serial sections of epoxy embedded specimens is described. Ribbons are cut from a block having the leading and trailing sides coated with contact cement. A scoop made from polyethylene tubing is used to remove a ribbon of sections from the boat of a glass or diamond knife and to transfer it to a pool of water on a microscope slide. Many ribbons (comprising hundreds of sections) can be mounted on a single slide. This method requires the construction of only one simple, inexpensive tool, the polyethylene scoop, and otherwise utilizes only items commonly available in the laboratory. |
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