| 无花果枝提取物体外诱导胃癌BGC-823细胞凋亡的研究 |
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| 引用本文： | 解美娜,李锋杰. 无花果枝提取物体外诱导胃癌BGC-823细胞凋亡的研究[J]. 天然产物研究与开发, 2010, 22(2) |
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| 作者姓名： | 解美娜 李锋杰 |
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| 作者单位： | 潍坊医学院医学研究实验中心,山东,261053 |
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| 基金项目： | 潍坊医学院青年教师启动基金 |
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| 摘 要： | 为探讨新鲜无花果枝提取物(FBE)对体外培养的人胃癌细胞株BGC-823增殖和凋亡的影响,用不同浓度FBE处理胃癌BGC-823细胞,采用细胞形态学观察,细胞活力测定(MTY法)及免疫组织化学法检测增殖细胞核抗原(PCNA)表达情况来评价FBE对BGC-823细胞体外增殖的影响,应用流式细胞术的膜联蛋白V/碘化丙啶技术(Annexin V/PI法)检测FBE诱导BGC-823细胞体外凋亡的情况.形态学观察发现中高剂量(＞0.5mg/mL)组处理4 h细胞出现明显凋亡,24 h检测到中高剂量(＞0.5 mg/mE)组细胞增殖活力和增殖细胞核抗原表达显著降低(P＜0.05),流式细胞仪检测到FBE处理4 h所有剂量组细胞平均凋亡率(9.76%,10.87%,14.29%,49.67%,71.37%)均高于对照组(6.1%)(P＜0.05或P＜0.01),以上作用效果呈现剂量依赖性.以上结果说明无花果枝提取物能够通过诱导胃癌BGC-823细胞凋亡从而抑制其体外的生长与增殖.
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| 关 键 词： | 无花果枝提取物 胃癌细胞 增殖 凋亡 |
Apoptosis of Human Gastric Cancer Line BGC-823 Induced by Fig Branch Extract |
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| XIE Mei-na,LI Feng-jie. Apoptosis of Human Gastric Cancer Line BGC-823 Induced by Fig Branch Extract[J]. Natural Product Research and Development, 2010, 22(2) |
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| Authors: | XIE Mei-na LI Feng-jie |
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| Affiliation: | XIE Mei-na,LI Feng-jieMedicine Experiment Center,Weifang Medical University,Sh,ong 261053,China |
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| Abstract: | To illustrate the growth inhibition and apoptosis in human gastric cancer BGC-823 cells treated with Fig Branch extract (FBE),morphologic changes of gastric cells were observed under an inversion microscope,colony formation inhibition and immunohistochemical staining detecting the expression of PCNA were used to reveal the effect of FBE on proliferation of BGC-823 cells,and flow cytometry was used to determine the apoptosis of cells by Annexin V/PI assay after treated with various concentration of FBE in vi... |
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| Keywords: | fig branch extract gastric cancer cell proliferation apoptosis |
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