Genetic analysis in fungi using restriction-enzyme-mediated integration |
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Authors: | Riggle P J Kumamoto C A |
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Institution: | Department of Molecular Biology, Tufts University School of Medicine, 136 Harrison Avenue, Boston MA 02111, USA. priggle@opal.tufts.edu |
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Abstract: | Restriction-enzyme-mediated integration (REMI), a method for generating nonhomologous integration of transforming DNA into the chromosomes of eukaryotic cells, has been used for insertion mutagenesis and other genetic studies in diverse organisms. Insertion mutations generated by REMI have facilitated the genetic dissection of developmental pathways in Dictyostelium discoidium and the isolation of virulence factors in several plant pathogenic fungi. Recent work indicates that REMI occurs by nonhomologous end joining. |
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