Changes in flavonoid content and tyrosinase inhibitory activity in kenaf leaf extract after far-infrared treatment |
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Authors: | Rho Ho Sik Ahn Soo Mi Lee Bum Chun Kim Myung Kyoo Ghimeray Amal Kumar Jin Cheng Wu Cho Dong Ha |
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Institution: | R&D Center, AmorePacific Corporation, Kyounggi-do, Yongin, Republic of Korea. thiocarbon@freechal.com |
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Abstract: | The tyrosinase inhibitory activity of ethanolic extract of kenaf (Hibiscus cannabinus L.) leaf was evaluated before and after subjecting it to far-infrared (FIR) irradiation. The main component of the extract was analyzed as kaempferitrin (kaempferol-3,7-O-α-dirhamnoside). Prior to FIR irradiation, no inhibitory activity of the extract was detected in a tyrosinase assay. However, after FIR irradiation for 1h at 60°C, significant tyrosinase inhibitory activity (IC(50)=3500 ppm) was observed in it. In HPLC analysis, derhamnosylation products (kaempferol, afzelin, and α-rhamnoisorobin) were detected. The inhibitory activity may be due to the existence of derhamnosylation products. This study demonstrated that FIR irradiation can be used as a convenient tool for deglycosylation of flavonoid glycoside. |
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