Photobleaching recovery studies of membrane events accompanying lectin stimulation of rabbit lymphocytes. |
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| Authors: | M D Leuther B G Barisas J S Peacock H Krakauer |
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| Institution: | E.A. Doisy Department of Biochemistry, St. Louis University St. Louis, Missouri 63104 USA;Department of Biochemistry, Washington State University Pullman, Washington 99164 USA |
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| Abstract: | Fluorescence photobleaching recovery methods reveal marked changes in lateral mobilities of rabbit lymphocyte membrane components during the course of stimulation with succinyl concanavalin A (S Con A). The diffusion constant of S Con A receptors on T lymphocytes falls from 1.6×10?10 cm2/sec to 6.5×10?11 cm2/sec within 4 hr after stimulation, remains constant for 14 hr, and returns to its former value. The mobility of B cell receptors similarly falls from 1.4×10?10 cm2/sec to 5.5×10?11 cm2/sec but regains its unstimulated value much more slowly. In contrast, a fluorescent phospholipid analog shows constant mobilities of 1.9×10?8 cm2/sec and 1.5×10?8 cm2/sec in T and B cells, respectively, throughout the experiment. |
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| Keywords: | FPR fluorescence photobleaching recovery Con A concanavalin A S Con A succinyl concanavalin A diI 3 3′-dioctadecylindocarbocyanine iodide TMR tetramethylrhodamine ATS antithymocyte serum HBSS Hanks balanced salt solution |
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